1 identification of two unknown species of bacteria by anna albrecht (microbiology 1420) introduction axonomy is defined as ―the science that studies organisms in order to arrange them into. Example, to identify the pathogen that is causing a disease in a patient, we find that there are many different species of bacteria present in the stool or tissue sample that we have obtained from the patient. The domain bacteria is very diverse, making it impossible to identify it without the use of staining and biochemical tests biochemical tests can test for a variety of characteristics such as shape, morphology, ph, etc that are specific to a certain type of bacteria. Unknown lab report april 25th, 2006 introduction the purpose of this lab was to identify two unknown bacteria cultures using various differential tests the identification of these unknown cultures was accomplished by separating and differentiating possible bacteria based on specific biochemical characteristics. Figure 2 shows the gram stain of the unknown bacteria and provides further proof that the bacteria is gram-positive because of the purple color it also reveals that the shape of the bacteria is bacillus, or rod shaped.
This study shows the importance of identifying unknown microorganisms by applying all the methods and techniques that were studied in the microbiology course throughout the semester by going through the steps to identify the microorganism, students began to comprehend infectious disease. Before going over the tests to identify an unknown bacterial species, we should remember some bases of manipulating bacteria it is important to always keep in mind that your unknown species is a potential pathogen. The identification of an unknown organism typically begins with a gram stain gram stains quickly tell not only if a bacterium is gram-positive or gram-negative, but.
Unknown lab report unknown number 116 micah schenck 4/29/2014 bio: 203604 introduction at the start of this semester in microbiology we have discussed the importance of being able to identify different bacteria. The strategy for identifying unknowns is to eliminate all bacteria giving contradictory results to those found in the identification matrix this is done automatically when you input results input a + result and all bacteria in the matrix with a - result are eliminated from consideration. Using targeted and metagenomic sequencing to identify and characterize pathogens in unknown respiratory disease outbreaks new coronaviruses can appear unexpectedly in different parts of the world, causing outbreaks of respiratory illness. Begin the process of identifying unknown bacteria by observing their physical characteristics, such as cell wall, shape and linkages use standard laboratory procedures, like cell staining, culturing and dna sequencing to further narrow down your identification. Gram negative or gram positive in order to identify my unknown species, i used good aseptic technique to separate bacteria on an isolation streak plate.
We are starting our unknown lab this week and we each will get 2 types of bacteria that we need to identify on our own i plan on doing a gram stain for each, then do streak plates w/selective media. The identification of unknown cultures is a practical application of a classification scheme, so that a new isolate can be recognized as a member of an existing species. The unknown bacterium could possibly be one of seven different bacteria that we have worked with during our laboratory course our instructor informed us that the unknown bacterium is either within the family enterobacteriaceae or pseudomonaceae. The purpose of this experiment was to perform a variety of tests to identify the bacteria contained in the unknown sample labeled number 15 the following are the tests that were used to identify the two different bacteria.
Bacterial flowcharts provide a visual aid to quick identification of bacterial species to create a flowchart, identify the common characteristics of the different groups of bacteria first to exclude non-relevant groups, and to more efficiently determine the bacteria present. Before attempting to identify an unknown bacterial species, the organism must be isolated in a ___ culture pure which of the following is the quickest way to isolate bacteria from a mixture. Tmcc's online microbiology resource center is designed to provide you with the resources to successfully complete the laboratory section of biology 251.
Identifying bacteria can lead to many new discoveries such as new species or the evolution of a known species in this experiment, an unknown bacterium was to be identified using a number of different tests using several different bacteria for comparison. Identification of unknown bacteria is one of the major responsibilities of the microbiologists samples of blood, tissue, food, water and cosmetics are examined daily in laboratories throughout the world for the presence of contaminating microorganisms in addition, industrial organisations are. Bacteria identification flow chart somewhat helpful if you were the ambitious one who used an environmental source for your unknown.
Unknown a, escherichia coli, was gram-stained to determine the bacterial shape when it was determined that it was a gram-negative rod due to its tube-shaped features and red staining, further testing needed to be done to eliminate potential organisms. Guide to the identification of an unknown bacterium - methods and report format pg 1 a standard part of nearly all lab courses in introductory microbiology is an activity wherein the student must use everything that has been learned in the course to identify and. Identifying the unknown bacterium was determined by separating and differentiating possible bacteria based on specific biochemical characteristics the differential tests used to identify the unknown cultures were gram staining, oxidase, indole test, urea test, and casein test.